Custom solutions & partnerships Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Keep up to date with the latest events Full event breakdown with abstracts, speakers, registration and more View global event calendar Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by recombinant technology for fast production Success from the first experiment – confirmed specificity through extensive validation Ethical standards compliant – production is animal-free Synthetic peptide within Human NLRP3 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.Database link: Q96P20 Run BLAST withRun BLAST with IHC-P: Human lung carcinoma tissue. Mouse intestine and embryo tissue. Rat spinal cord and kidney tissue. WB: Raji, SiHa and MDA-MB-231 cell lysate, mouse spleen and thymus tissue lysate. General notes The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257).The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer pH: 7.2Preservative: 0.09% Sodium azideConstituents: 50% Glycerol (glycerin, glycerine), 1% BSA The Abpromise guarantee Our Abpromise guarantee covers the use of ab214185 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Flow Cyt 1/1000. IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 1/200. WB (1) 1/100 - 1/1000. Stimulation may be required to allow detection of the NLRP3 protein in most samples.There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs. IHC-P1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 1/200. WB1/100 - 1/1000. Stimulation may be required to allow detection of the NLRP3 protein in most samples.There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs. Function May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Tissue specificity Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. Involvement in disease Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Sequence similarities Belongs to the NLRP family.Contains 1 DAPIN domain.Contains 9 LRR (leucine-rich) repeats.Contains 1 NACHT domain. Cold autoinflammatory syndrome 1 antibody Cold autoinflammatory syndrome 1 protein antibody Cryopyrin antibody Familial cold autoinflammatory syndrome antibody FCAS antibody FCU antibody LRR and PYD domains-containing protein 3 antibody Muckle-Wells syndrome antibody MWS antibody NACHT antibody NACHT LRR and PYD containing protein 3 antibody NALP 3 antibody NALP3 antibody NALP3_HUMAN antibody NLR family pyrin domain containing 3 antibody NLRP3 antibody PYPAF 1 antibody PYPAF1 antibody PYRIN containing APAF1 like protein 1 antibody PYRIN-containing APAF1-like protein 1 antibody see all Immunohistochemical analysis of formalin/PFA-fixed paraffin-embeddedmouse intestinetissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining. All lanes : Anti-NLRP3 antibody (ab214185) at 1/300 dilutionLane 1 : Raji (human Burkitt\'s lymphoma cell line) cell lysateLane 2 : MDA-MB-231 (human breast adenocarcinoma cell line/0 cell lysateSecondaryAll lanes : HRP conjugated goat anti-rabbit IgG at 1/5000 dilution Immunohistochemical analysis of formalin/PFA-fixed paraffin-embeddedmouse embryo tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining. Anti-NLRP3 antibody (ab214185) at 1/1000 dilution + Rat thymus lysateSecondarySecondary conjugated at 1/20000 dilution All lanes : Anti-NLRP3 antibody (ab214185) at 1/1000 dilutionLane 1 : Mouse spleen tissue lysateLane 2 : Mouse thymus tissue lysateSecondaryAll lanes : Conjugated secondary antibody at 1/20000 dilution Immunohistochemical analysis of formalin/PFA-fixed paraffin-embeddedhuman lung carcinomatissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining. Immunohistochemical analysis of formalin/PFA-fixed paraffin-embeddedmouse intestinetissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining. Flow cytometry analysis of human Jurkat cell line labelled for NLRP3 using ab214185 at 1/1000 dilution. Acquisitions of 20,000 events were performed. Anti-NLRP3 antibody (ab214185) at 1/300 dilution + SiHa (Human cervical carcinoma cell line) cell lysateSecondaryHRP-conjugated goat anti-rabbit IgG Publishing research using ab214185? Please let us know so that we can cite the reference in this datasheet. ab214185 has been referenced in 158 publications. Diao Y Clematichinenoside AR Alleviates Foam Cell Formation and the Inflammatory Response in Ox-LDL-Induced RAW264.7 Cells by Activating Autophagy. Inflammation 44:758-768 (2021).PubMed: 33151398 Lv Q et al. Uric acid drives intestinal barrier dysfunction through TSPO-mediated NLRP3 inflammasome activation. Inflamm Res 70:127-137 (2021).PubMed: 33074353 Hou N et al. Sophocarpine attenuates septic liver injury through suppression of the NLRP3 inflammasome via autophagy-mediated degradation. Exp Ther Med 20:249 (2020).PubMed: 33178347 Li X et al. SENP7 knockdown inhibited pyroptosis and NF-?B/NLRP3 inflammasome pathway activation in Raw 264.7 cells. Sci Rep 10:16265 (2020).PubMed: 33004957 Wu ZM et al. Icariin alleviates rheumatoid arthritis via regulating miR-223-3p/NLRP3 signalling axis. Autoimmunity 53:450-458 (2020).PubMed: 33084415 View all Publications for this product Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 22 CPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com