Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 8 | 4.8% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 3 | 5.6% |
Sample type | Average % | Range |
---|---|---|
Cell culture supernatant | 98 | 96%- 100% |
Serum | 103 | 101%- 105% |
Hep Plasma | 100 | 99%- 101% |
EDTA Plasma | 93 | 90%- 96% |
Cit plasma | 86 | 84%- 88% |
Human IL-1 beta (Interleukin 1 beta) ELISA kit is a single-wash sandwich ELISA designed for the quantitative measurement of IL-1 beta protein in human serum, plasma, and cell culture supernatants.It uses our proprietary SimpleStep ELISA® technology. Quantitatehuman IL-1 beta with 5.64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human IL-1beta protein in serum, plasma, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY
Recombinant mouse IL-1beta and human IL-1 receptor antagonist were prepared at 50 ng/mL and 225 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE
Recombinant human Interleukin-1 receptor type 1 was prepared at 50 ng/mL and 225 pg/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY
This kit recognizes human IL-1beta protein.
Other species reactivity was determined by measuring serum samples of various species, interpolating the IL-1beta protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the IL-1beta protein concentration in human serum assayed at the same dilution.
Reactivity < 3%="" was="" determined="" for="" the="" following="" species:="" mouse,="" rat,="">
Other species reactivity not determined.
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Components | 1 x 96 tests |
---|---|
10X Human IL-1beta Capture Antibody | 1 x 600µl |
10X Human IL-1beta Detector Antibody | 1 x 600µl |
10X Wash Buffer PT (ab206977) | 1 x 20ml |
Antibody Diluent 4BI | 1 x 6ml |
Human IL-1beta Lyophilized Recombinant Protein (ab9617) | 2 vials |
Plate Seals | 1 unit |
Sample Diluent NS (ab193972) | 1 x 50ml |
SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
Stop Solution | 1 x 12ml |
TMBDevelopment Solution | 1 x 12ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
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