Overview
Product name
Human Galectin-3 ELISA Kit (LGALS3)See all Galectin 3 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 8 3.4% Inter-assay Sample n Mean SD CV% Serum 3 1.6% Sample type
Cell culture supernatant, Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
13.3 pg/mlRange
58.8 pg/ml -2000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 109%- 120% Serum 113 107%- 120% Cell Lysate 109 103%- 118% Cell culture media 93 91%- 95% Hep Plasma 109 107%- 111% EDTA Plasma 113 111%- 116% Cit plasma 110 106%- 114% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human Galectin-3 ELISA Kit (LGALS3) (ab269555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in cell lysate, serum, cell culture media, cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-3 (LGALS3) with 13.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Galectin-3 (LGALS3) Capture Antibody 1 x 600µl 10X Human Galectin-3 (LGALS3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Galectin-3 (LGALS3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Neuroscience
- Cell Adhesion Proteins
- Nuclear Proteins
- Cell Biology
- Cell Cycle
- Cell differentiation
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.Sequence similarities
Contains 1 galectin domain.Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.- Information by UniProt
Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see allDatabase links
- Entrez Gene:3958 Human
- Omim:153619 Human
- SwissProt:P17931 Human
- Unigene:531081 Human
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