Overview
Product name
MitoBiogenesis™ Western Blot CocktailSpecies reactivity
Reacts with:Mouse, Rat, Cow, HumanProduct overview
The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.
Cocktail Antibodies:
Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse Beta actin [mAbcam 8224] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Notes
Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Related products
Review themitochondrial assay guide, or the fullmetabolism assay guideto learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 100 µg Antibody Cocktail 1 x 200µl Research areas
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Microfilaments
- Actin etc
- Actin
- Signal Transduction
- Signaling Pathway
- Lipid Signaling
- Prostaglandins
- Signal Transduction
- Metabolism
- Energy Metabolism
- Signal Transduction
- Metabolism
- Mitochondrial
- Signal Transduction
- Metabolism
- Lipid metabolism
- Cell Biology
- Other Antibodies
- Oxidative Stress
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of carbohydrates
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Integration of energy metabolism
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Carbohydrate metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Metabolism
- Pathways and Processes
- Redox metabolism
- Oxidative stress
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Oxidative phosphorylation
- Complex II
- Metabolism
- Types of disease
- Cancer
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- MitoBiogenesis
Database links
- SwissProt:P23219 Human
- SwissProt:P60709 Human
- SwissProt:P31040 Human
Abcam 位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂，并不断添加，供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的 MitoSciences 公司，加强了免疫分析方面的产品供应；同年也并购了英国的 Ascent Scientific 公司，开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司，成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴，为所有产品提供技术支持来使客户获得预期的结果，为提供高质量的抗体来指向尽可能多的靶蛋白，尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域，包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。
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