Abcam/Recombinant Human HIF-1 alpha protein (ab154478)/1/ab154478,Abcam,,Abcam,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Abcam/Recombinant Human HIF-1 alpha protein (ab154478)/1/ab154478

产品编号：ab154478

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Abcam

公司：Abcam

公司分类：

商品介绍

Product name
Recombinant Human HIF-1 alpha proteinSee all HIF-1 alpha proteins and peptides
Purity
>75% SDS-PAGE.ab154478 was purified by Ni chromatography and sterile filtered.
Expression system
Escherichia coli
Accession
Q16665-2
Protein length
Full length protein
Animal free
No
Nature
Recombinant
- Species
  Human
- Sequence
  MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEY QGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQL KEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPD LGTENLYFQGMEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYEL AHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNC FYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCD HEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWK VLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSK TFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKT HHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNY VVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKL KKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPN EKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFT MPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLF AEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASP ESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIA SPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNV LSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIG
- Predicted molecular weight
  100 kDa including tags
- Amino acids
  1 to 735
- Tags
  His tag N-Terminus

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- Anti-6X His tag® antibody (ab9108)

Our Abpromise guarantee covers the use ofab154478in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications
ELISA
Western blot
SDS-PAGE
Form
Liquid
Additional notes
Product was previously marketed under the MitoSciences sub-brand.
Concentration information loading...

Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00Constituents: 0.61% Tris, 10% Glycerol, 0.88% Sodium chloride

Alternative names
- ARNT interacting protein
- ARNT-interacting protein
- Basic helix loop helix PAS protein MOP1
- Basic-helix-loop-helix-PAS protein MOP1
- bHLHe78
- Class E basic helix-loop-helix protein 78
- HIF 1A
- HIF 1alpha
- HIF-1-alpha
- HIF-1alpha
- HIF-alpha
- HIF1
- HIF1 A
- HIF1 Alpha
- HIF1-alpha
- HIF1A
- HIF1A_HUMAN
- hifla
- Hypoxia inducible factor 1 alpha
- Hypoxia inducible factor 1 alpha isoform I.3
- Hypoxia inducible factor 1 alpha subunit
- Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor
- Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor)
- Hypoxia inducible factor1alpha
- Hypoxia-inducible factor 1-alpha
- Member of PAS protein 1
- Member of PAS superfamily 1
- Member of the PAS Superfamily 1
- MOP 1
- MOP1
- PAS domain-containing protein 8
- PASD 8
- PASD8
see all
Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5"-[AG]CGTG-3" within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Tissue specificity
Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.
Domain
Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
Post-translationalmodifications
In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.Requires phosphorylation for DNA-binding.Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
Target information above from: UniProt accessionQ16665The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).
Information by UniProt

品牌介绍

Abcam 位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂，并不断添加，供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的 MitoSciences 公司，加强了免疫分析方面的产品供应；同年也并购了英国的 Ascent Scientific 公司，开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司，成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴，为所有产品提供技术支持来使客户获得预期的结果，为提供高质量的抗体来指向尽可能多的靶蛋白，尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域，包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。

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