Abcam/Recombinant human PLK1 protein (ab51426)/1/ab51426,Abcam,,Abcam,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Abcam/Recombinant human PLK1 protein (ab51426)/1/ab51426

产品编号：ab51426

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Abcam

公司：Abcam

公司分类：

商品介绍

Product name
Recombinant human PLK1 proteinSee all PLK1 proteins and peptides
Biological activity
Specific activity 17nmol/min/mg.
Expression system
Baculovirus infected Sf9 cells
Protein length
Full length protein
Animal free
No
Nature
Recombinant
- Species
  Human
- Tags
  His tag N-Terminus

Related Products
- Anti-PLK1 antibody [35-206] (ab17056)
- Anti-PLK1 antibody [36-298] (ab17057)
Substrate reagent
- Native Cow Casein protein (ab91090)

Our Abpromise guarantee covers the use ofab51426in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications
Western blot
Functional Studies
SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Form
Liquid
Additional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...

Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.

Alternative names
- Cell cycle regulated protein kinase
- PLK
- PLK 1
- PLK-1
- plk1
- PLK1_HUMAN
- Polo like kinase 1
- Polo-like kinase 1
- Serine/threonine protein kinase 13
- Serine/threonine protein kinase PLK1
- Serine/threonine-protein kinase 13
- Serine/threonine-protein kinase PLK1
- STPK 13
- STPK13
see all
Function
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
Tissue specificity
Placenta and colon.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.Contains 2 POLO box domains.Contains 1 protein kinase domain.
Developmental stage
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
Post-translationalmodifications
Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
Target information above from: UniProt accessionP53350The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).
Information by UniProt

品牌介绍

Abcam 位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂，并不断添加，供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的 MitoSciences 公司，加强了免疫分析方面的产品供应；同年也并购了英国的 Ascent Scientific 公司，开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司，成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴，为所有产品提供技术支持来使客户获得预期的结果，为提供高质量的抗体来指向尽可能多的靶蛋白，尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域，包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。

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