Overview
Product name
Rat IL-2 ELISA KitSee all IL-2 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 3 4% Inter-assay Sample n Mean SD CV% Serum 5 4.2% Sample type
Serum, Cell culture media, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
4.9 pg/mlRange
31.25 pg/ml -2000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 93 89%- 100% Tissue Extracts 45 43%- 48% Cell culture media 108 99%- 113% Cit plasma 86 84%- 88% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:RatProduct overview
Rat IL-2 ELISA Kit (ab221834) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in serum, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-2 with 4.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.
Platform
Microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Rat IL-2 Capture Antibody 1 x 600µl 10X Rat IL-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Rat IL-2 Lyophilized Recombinant Protein 2 vials Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Immunology
- Innate Immunity
- Cytokines
- Interleukins
Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.Sequence similarities
Belongs to the IL-2 family.Cellular localization
Secreted.- Information by UniProt
Alternative names
- Aldesleukin
- IL 2
- IL-2
see allDatabase links
- Entrez Gene:116562 Rat
- SwissProt:P17108 Rat
- Unigene:9871 Rat
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