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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Abcam/Recombinant human FGF2 protein (Active) (ab155734)/Active) (ab155734
产品编号:Active)(ab155734
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Abcam
公      司:Abcam
公司分类:
Abcam/Recombinant human FGF2 protein (Active) (ab155734)/Active) (ab155734
商品介绍

Description

  • Product name

    Recombinant human FGF2 protein (Active)See all FGF2 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.Immobilized ab155734at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag (ab220538) with a linear range of 4-125 ng/mL.

    Measured by its binding ability in a functional ELISA.Immobilized ab155734at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag with a linear range of 1-39 ng/mL.

    Measured by its binding ability in an SPR assay. Immobilized ab155734 on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200).

    Measured by its binding ability in a BLI assay.Loaded Biotinylated Heparin on SA Biosensor can bind ab155734 with an affinity constant of 1.88nM as determined in BLI assay (ForteBio Octet Red96e).

  • Purity

    >95% SDS-PAGE.ab155734 was lyophilised from 0.22 µm filtered solution.
  • Endotoxin level

    <>
  • Expression system

    Escherichia coli
  • Accession

    P09038
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPH IKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLES NNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
    • Predicted molecular weight

      17 kDa
    • Amino acids

      143 to 288

Associated products

    Specifications

    Our Abpromise guarantee covers the use ofab155734in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      The product is stable after storage at:

      • -20 to -70oC for 12 months in lyophilized state;
      • -70oC for 3 months under sterile conditions after reconstitution.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40Constituents: 95% PBS, 5% Trehalose

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

    General Info

    • Alternative names

      • Basic fibroblast growth factor
      • Basic fibroblast growth factor bFGF
      • BFGF
      • FGF 2
      • FGF B
      • FGF-2
      • Fgf2
      • FGF2 basic
      • FGF2_HUMAN
      • FGFB
      • Fibroblast growth factor 2
      • Fibroblast growth factor 2 (basic)
      • Fibroblast growth factor, basic
      • HBGF 2
      • HBGF-2
      • HBGF2
      • HBGH 2
      • HBGH2
      • Heparin binding growth factor 2 precursor
      • Heparin-binding growth factor 2
      • Prostatropin
      see all
    • Function

      Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
    • Tissue specificity

      Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
    • Sequence similarities

      Belongs to the heparin-binding growth factors family.
    • Post-translationalmodifications

      Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
    • Cellular localization

      Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
    • Information by UniProt
    品牌介绍

    Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
    为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
    Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
    Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。

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