Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Media | 8 | 4.8% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Media | 3 | 5.6% |
Sample type | Average % | Range |
---|---|---|
Cell culture supernatant | 98 | 96%- 100% |
Serum | 103 | 101%- 105% |
Hep Plasma | 100 | 99%- 101% |
EDTA Plasma | 93 | 90%- 96% |
Cit plasma | 86 | 84%- 88% |
IL-1 beta in vitro CatchPoint®ELISAkit is designed for the quantitative measurement of IL-1 beta protein in human serum, plasma and cell culture supernatants.
This CatchPoint ELISA kit has beenoptimized for Molecular Devices Microplate Readers. Clickherefor a list of recommended Microplate Readers.If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint ELISA Kits is available with all the protocol and analysis settings atwww.softmaxpro.org.
The CatchPoint®ELISAemploys an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPointHRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Components | 1 x 96 tests |
---|---|
100X Stoplight Red Substrate | 1 x 120µl |
10X Human IL-1beta Capture Antibody | 1 x 600µl |
10X Human IL-1beta Detector Antibody | 1 x 600µl |
10X Wash Buffer PT (ab206977) | 1 x 20ml |
500X Hydrogen Peroxide (H2O2, 3%) | 1 x 50µl |
Antibody Diluent 4BI | 1 x 6ml |
Human IL-1beta Lyophilized Recombinant Protein (ab9617) | 2 vials |
Plate Seals | 1 unit |
Sample Diluent NS (ab193972) | 1 x 50ml |
SimpleStep Pre-Coated Black 96-Well Microplate | 1 unit |
Stoplight Red Substrate Buffer | 1 x 12ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。