Overview
Product name
Human EMMPRIN ELISA KitSee all CD147 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Urine 8 2.5% Inter-assay Sample n Mean SD CV% Urine 3 3% Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
3.88 pg/mlRange
31.3 pg/ml -200 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 112 111%- 113% Saliva 95 94%- 95% Urine 98 97%- 98% Serum 97 91%- 102% Cell culture media 103 99%- 110% Hep Plasma 115 113%- 117% EDTA Plasma 108 107%- 109% Cit plasma 102 100%- 105% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanDoes not react with:CowProduct overview
Human EMMPRIN ELISA Kit (ab219631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EMMPRIN with 3.88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Extracellular matrix metalloproteinase inducer (EMMPRIN), also known as Basigin (BSG), and CD147 is a 363-amino acid protein encoded by the gene BSG. EMMPRIN is a member of the immunoglobin superfamily, and targets the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane. EMMPRIN is the determinant for the Oκ blood group system, and has been demonstrated to be a receptor on red blood cells for the malaria parasite, Plasmodium falciparum. In addition to its metalloproteinase-inducing ability, EMMPRIN is thought to regulate several other functions, including spermatogenesis, embryo implantation, neural network formation, and tumor progression. EMMPRIN also has several ligands including cyclophilin proteins, caveolin-1, and integrins.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human EMMPRIN Capture Antibody 1 x 600µl 10X Human EMMPRIN Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human EMMPRIN Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Immunology
- Cell Type Markers
- CD
- Endothelial Cells
- Neuroscience
- Sensory System
- Visual system
- Neuroscience
- Neurology process
- Neurogenesis
- Cancer
- Invasion/microenvironment
- ECM
- Extracellular matrix
- Other
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of carbohydrates
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Carbohydrate metabolism
- Cardiovascular
- Angiogenesis
- Endothelial Cell Markers
Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Post-translationalmodifications
N-glycosylated.Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.- Information by UniProt
Alternative names
- 5A11 antigen
- 5F7
- BASI_HUMAN
see allDatabase links
- Entrez Gene:682 Human
- Omim:109480 Human
- SwissProt:P35613 Human
- Unigene:501293 Human
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