Overview
Product name
Human Caspase-7 ELISA KitSee all Caspase-7 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Extract 11 4% Sample type
Cell LysateAssay type
Sandwich (quantitative)Sensitivity
41 pg/mlRange
93.75 pg/ml -6000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell Lysate 104 94%- 118% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human Caspase-7 ELISA kit (ab274396) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Caspase-7 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Caspase-7 with 41 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Human Caspase-7 is a cytoplasmic cysteine protease involved in the activation cascade of caspases responsible for execution of apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs). Caspase-7 cleaves poly(ADP-ribose) polymerase (PARP). Caspase-7 overexpression promotes programmed cell death. Caspase-7 is expressed as an inactive pro-form (pro caspase-7). In apoptosis, the pro caspase-7 is activated by proteolytic cleavages at Asp23-Ala24, Asp198-Ser199 and Asp206-Ala207 bonds catalyzed by granzyme B and caspase-10, generating two subunits p20 and p11 that are assembled into hetero-tetrameric active enzyme. This kit does not differentiate between the inactive and active forms Caspase-7; it measures Caspase-7 whether in pro form or cleaved form.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Caspase-7 Capture Antibody 1 x 600µl 10X Human Caspase-7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody DiluentCPI - HAMA Blocker (ab193969) 1 x 6ml Human Caspase-7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cell Biology
- Apoptosis
- Intracellular
- Caspases etc
- Caspases
- Cancer
- Invasion/microenvironment
- Apoptosis
- Caspases
- Cell Biology
- Proteolysis / Ubiquitin
- Proteolytic enzymes
- Other proteases
- Metabolism
- Pathways and Processes
- Metabolism processes
- Apoptosis
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- Caspases
- Cancer
- Cell Death
- Apoptosis
- Metabolism
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a "216-Asp--Gly-217" bond. Overexpression promotes programmed cell death.Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.Sequence similarities
Belongs to the peptidase C14A family.Post-translationalmodifications
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.Cellular localization
Cytoplasm.- Information by UniProt
Alternative names
- Apoptotic protease Mch-3
- Apoptotic protease MCH3
- CASP-7
see allDatabase links
- Entrez Gene:840 Human
- Omim:601761 Human
- SwissProt:P55210 Human
- Unigene:9216 Human
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