The FirePlex®miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex®Analysis Workbench software.
Our multiplex miRNA assays use our FirePlex®particle technology to profile up to 65 miRNA directly from 5 micron sections of FFPE or fresh-frozen tissue, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex®hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.
The FirePlex®miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay"s multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 5 micron of tissue sample.
For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-assays/fireplex-mirna-assays
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
Components | 1 x 96 tests |
---|---|
10x Rinse Buffer A | 1 x 30ml |
1x Hyb Buffer (Hybridization Buffer) | 1 x 25ml |
2x Rinse Buffer B | 1 x 33ml |
50x Labeling Buffer | 1 x 200µl |
5x Reporter Solution | 1 x 4ml |
Catch Plate | 2 units |
Digest Buffer | 1 x 30ml |
dNTP mix | 1 x 120µl |
FFPE/Tissue Control | 1 unit |
Filter Plate | 2 units |
Labeling Diluent | 1 x 30ml |
Labeling Enzyme | 1 x 50µl |
PCR Buffer | 1 x 2ml |
PCR Enzyme | 1 x 60µl |
Primer Mix | 1 x 250µl |
Protease Mix | 1 x 2ml |
RNA Control | 1 x 50µl |
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为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
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