FirePlex® miRNA Assay Core Reagent Kit V2 - Biofluid (ab218342) contains qualified reagents for the use of our FirePlex® miRNA multiplex assays which detect up to 65 literature curated miRNA in only 10 µL of biofluid samples or100 pg purified total RNA, without the need for RNA purification.

FirePlex® miRNA Assay Core Reagent Kit V2 - Biofluid (ab218342) requires the purchase of either:

- Oncology miRNA Panel (ab218367)- Cardiology miRNA Panel (ab218368)- Immunology miRNA Panel (ab218369)- Liver Tox miRNA Panel (ab218370)- Neurology miRNA Panel (ab218371)- Kidney Toxicity miRNA Panel (ab219508)- Cardiac Toxicity miRNA Panel (ab219509)- Pancreatic Toxicity miRNA Panel (ab219510)

NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.

The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex^® hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.

The FirePlex^®miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay"s multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 10 µLof biofluid, and has been validated for numerous sample types including plasma, serum, exosomes, urine and saliva.

Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.

Our integrated free FirePlexAnalysis Workbench software enables easy and rapid data analysis, visualization, and export in under ten minutes, and includes key features such as standard curve analysis and publication-quality heat maps and graphs.