• Product name

  Recombinant human SIRP alpha protein (Fc Chimera Active)See all SIRP alpha proteins and peptides

• Biological activity

  Measured by its binding ability in a functional ELISA. Recombinant human CD47 protein (Active) (ab174029) at 2µg/mL (100µL/well) can bind ab221342 with a linear range of 4-31 ng/mL.

  Measured by its binding ability in a functional ELISA. Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into ab221342: Recombinant human CD47 protein (Fc Chimera Active) (Biotin) (ab246018) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006µg/mL.

  Measured by its binding ability inFACS.The binding ofab221342 to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 µg/ml. IC50=0.1318 µg/ml.

  Measured by its binding ability inFACS. Recombinantab221342 can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 µg/ml.

• Purity

  >95% SDS-PAGE.>90% pure as determined by SEC-HPLC.

• Endotoxin level

  <>

• Expression system

  HEK 293 cells

• Accession

  P78324

• Protein length

  Protein fragment

• Animal free

  No

• Nature

  Recombinant

• • Species

    Human

  • Sequence

    EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIY NQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPD DVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDI TLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEV AHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYP QRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLT CQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

  • Predicted molecular weight

    64 kDa including tags

  • Amino acids

    31 to 370

  • Tags

    Fc tag C-Terminus

  • Additional sequence information

    This protein carries a mouse IgG1 Fc tag at the C-terminus (Val 98-Lys 324; AAK53870.1). (NP_001035111).

• Stability and Storage

  Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  pH: 7.4Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• Reconstitution
  Reconstitute with sterile deionized water to a concentration of 500 µg/ml.

Abcam 位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂，并不断添加，供应予全球百多个国家。
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Abcam 的目标是给世界上最好的抗体建立最大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴，为所有产品提供技术支持来使客户获得预期的结果，为提供高质量的抗体来指向尽可能多的靶蛋白，尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域，包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。