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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Abcam/Native human IgE protein (Azide free) (ab65866)/1/ab65866
产品编号:ab65866
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Abcam
公      司:Abcam
公司分类:
Abcam/Native human IgE protein (Azide free) (ab65866)/1/ab65866
商品介绍

Description

  • Product name

    Native human IgE protein (Azide free)See all IgE proteins and peptides
  • Biological activity

    1 mg ofab65866 is 380,000± 50,000 IU/ml.

    IgE has been used in a variety of cell stimulation assays, including the following cell types:

    - Human airway smooth muscle (HASM) cells

    - mast cells (MC)

    - Human bronchial/tracheal smooth muscle (B/TSM) cells

    - acute myelogenous leukemia (AML) blasts

    - Human neutrophils

  • Purity

    >90% SDS-PAGE.Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
  • Endotoxin level

    <>
  • Expression system

    Native
  • Accession

    P0DOX4P0DOX7
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Amino Acid Sequence 1

    • Species

      Human
    • Additional sequence information

      Observed band of ~185kDa on SDS-PAGE
    • Amino Acid Sequence 2

    • Species

      Human

Associated products

    Specifications

    Our Abpromise guarantee covers the use ofab65866in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      IHC - Wholemount

      Western blot

      ELISA

      Immunocytochemistry

    • Form

      Liquid
    • Additional notes

      This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

      This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

      - Less batch-to-batch variation

      - Higher degree of purity (>90%)

      - A more uniform product (monoclonal and not polyclonal)

      Low endotoxin levels below < 25="" eu/mg="" allow="" use="" of="" ige="" protein="" in="" cellular="" immunology="" and="" in="" cell="" proliferation="">

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

      pH: 7.40Constituents: 0.00017% PBS, 0.00087% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Igh2
      • IGHE
      • IGHEP1
      • Immunoglobulin heavy constant epsilon
    • Relevance

      IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
    • Cellular localization

      Cell Membrane
    品牌介绍

    Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
    为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
    Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
    Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。

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