Human ALT ELISA Kit (ab234578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALT with 47.34 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only.

Tissue extract samples have not been tested with this kit.

This kit is incompatible with plasma- heparin and cell extract samples.

SPECIES REACTIVITYThis kit recognizes human ALT protein. Other species reactivity not determined. Please contact our Technical Support team for more information.

• Signal Transduction
• Metabolism
• Amino Acids

• Metabolism
• Pathways and Processes
• Metabolic signaling pathways
• Amino acid metabolism