Overview
Product name
Mouse C Reactive Protein ELISA KitSee all C Reactive Protein kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 5 2.4% Inter-assay Sample n Mean SD CV% Serum 3 2.1% Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
17 pg/mlRange
156.25 pg/ml -10000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 111 109%- 113% Tissue Extracts 115 103%- 122% Hep Plasma 113 110%- 117% EDTA Plasma 107 103%- 109% Cit plasma 108 90%- 117% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:MouseDoes not react with:CowProduct overview
Mouse C Reactive Protein ELISA Kit (ab222511) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C Reactive Protein protein in cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse C Reactive Protein with 31 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
C Reactive Protein displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. C Reactive Protein can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. C Reactive Protein is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. C Reactive Protein binds 2 calcium ions per subunit. The concentration of C Reactive Protein in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1and IL6//interleukin-6.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse C Reactive Protein Capture Antibody Concentrate 1 x 22µl Mouse C Reactive Protein DetectorAntibody Concentrate 1 x 22µl Mouse C Reactive Protein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Immunology
- Innate Immunity
- Macrophage / Inflamm.
- Cardiovascular
- Blood
- Acute Phase Reactants
- Immunology
- Innate Immunity
- Complement
- Classical Pathway
- Epigenetics and Nuclear Signaling
- Chromatin Binding Proteins
- DNA / RNA binding
- Cardiovascular
- Atherosclerosis
- Vascular Inflammation
- Inflammatory mediators
- Cardiovascular
- Heart
- Apoptosis
- Metabolism
- Types of disease
- Metabolic disorders
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.Tissue specificity
Found in plasma.Sequence similarities
Belongs to the pentaxin family.Contains 1 pentaxin domain.Cellular localization
Secreted.- Information by UniProt
Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see allDatabase links
- Entrez Gene:12944 Mouse
- SwissProt:P14847 Mouse
- Unigene:28767 Mouse
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