Sample | n | Mean | SD | CV% |
---|---|---|---|---|
rat serum | 5 | 1.6% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
rat serum | 3 | 3.3% |
Sample type | Average % | Range |
---|---|---|
Urine | 80 | 69%- 88% |
Serum | 100 | 94%- 105% |
Cell culture media | 99 | 98%- 102% |
Cit plasma | 85 | 84%- 87% |
serum free media | 95 | 91%- 98% |
Rat PAI1 ELISA Kit (SERPINE1) (ab201283) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat PAI1 (SERPINE1) with 159 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.
Components | 1 x 96 tests |
---|---|
10X Rat PAI1 Capture Antibody | 1 x 600µl |
10X Rat PAI1 Detector Antibody | 1 x 600µl |
10X Wash Buffer PT (ab206977) | 1 x 20ml |
Antibody Diluent 4BI | 1 x 6ml |
Plate Seals | 1 unit |
Rat PAI1 Lyophilized Recombinant Protein | 2 vials |
Sample Diluent NS (ab193972) | 1 x 50ml |
SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
Stop Solution | 1 x 12ml |
TMBDevelopment Solution | 1 x 12ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。