Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Serum | 3 | 5% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Serum | 5 | 8.3% |
Sample type | Average % | Range |
---|---|---|
Cell culture supernatant | 102 | 90%- 117% |
Milk | 102 | 91%- 111% |
Serum | 93 | 87%- 102% |
Cell culture extracts | 89 | 80%- 105% |
Tissue Extracts | 90 | 80%- 131% |
EDTA Plasma | 86 | 81%- 94% |
Cit plasma | 99 | 87%- 120% |
Human Fibronectin ELISA Kit (ab219046) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, cit plasma, edta plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibronectin with 20.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITYThis kit recognizes both native and recombinant human Fibronectin protein in serum, plasma (citrate and EDTA), milk, cell culture supernatant, and cell and tissue extract samples only.
CROSS REACTIVITY Recombinant human Anastelin was prepared within the working range of the assay and assayed for cross reactivity. No cross-reactivity was observed.SPECIES REACTIVITYThis kit recognizes human Fibronectin protein.
Other species reactivity was determined by measuring 1/100,000 serum (mouse, rat, cow) or plasma (Rhesus macaque) samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution.
Fibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis and nerve regeneration. Differential expression of fibronectin is seen in coronary heart disease, glomerulopathy and tumor cell metastasis. The protein contains binding sites for collagen, heparin and fibrin and is a specific ligand for several integrin adhesion receptors. Fibronectin exists as a dimer or multimer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Components | 1 x 96 tests |
---|---|
10X Wash Buffer PT (ab206977) | 1 x 20ml |
50X Cell Extraction Enhancer Solution (ab193971) | 1 x 1ml |
5X Cell Extraction Buffer PTR (ab193970) | 1 x 10ml |
Antibody Diluent 4BR | 1 x 6ml |
10X Human Fibronectin Capture Antibody | 1 x 600µl |
10X Human Fibronectin Detector Antibody | 1 x 600µl |
Human Fibronectin Lyophilized Recombinant Protein | 2 vials |
Plate Seals | 1 unit |
Sample Diluent NS (ab193972) | 1 x 50ml |
SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
Stop Solution | 1 x 12ml |
TMBDevelopment Solution | 1 x 12ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。