Overview
Product name
S100B ELISA KitSee all S100 beta kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Protein 6 2.5% Sample type
Tissue ExtractsAssay type
Sandwich (quantitative)Sensitivity
139 pg/mlRange
0.31 ng/ml -20 ng/mlRecovery
Sample specific recovery Sample type Average % Range Tissue Extracts 97 92%- 100% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
S100B ELISA Kit (ab234573) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100B protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate S100B with 139 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human S100B Capture Antibody 1 x 600µl 10X Human S100B Detector Antibody 1 x 600µl Human S100B Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cell Biology
- Cell Cycle
- Cell differentiation
- Epigenetics and Nuclear Signaling
- Cell cycle
- Cell Differentiation
- Stem Cells
- Neural Stem Cells
- Glial Restricted Lineage
- Astrocyte
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.Sequence similarities
Belongs to the S-101 family.Contains 2 EF-hand domains.Cellular localization
Cytoplasm. Nucleus.- Information by UniProt
Alternative names
- NEF
- Protein S100 B
- Protein S100-B
see allDatabase links
- Entrez Gene:6285 Human
- Entrez Gene:20203 Mouse
- Entrez Gene:25742 Rat
- Omim:176990 Human
- SwissProt:P04271 Human
- SwissProt:P50114 Mouse
- SwissProt:P04631 Rat
- Unigene:422181 Human
see all
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