Overview
Product name
Human MRP1 ELISA Kit (ABCC1)See all MRP1 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Extract 8 2.9% Sample type
Tissue Extracts, Cell LysateAssay type
Sandwich (quantitative)Sensitivity
0.151 ng/mlRange
0.63 ng/ml -40 ng/mlRecovery
Sample specific recovery Sample type Average % Range Tissue Extracts 95 94%- 96% Cell Lysate 94 91%- 97% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
HumanMRP1 ELISA kit (ab272211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement ofMRP1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. QuantitateHumanMRP1 with 151 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
MRP1 (Multidrug Resistance-associated Protein 1), also known as ABCC1 (ATP-Binding Cassette sub-family C member 1), mediates export of organic anions and drugs from the cytoplasm. It mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. MRP1 confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human MRP1 (ABCC1) Capture Antibody 1 x 600µl 10X Human MRP1 (ABCC1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human MRP1 (ABCC1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Signal Transduction
- Metabolism
- Plasma Membrane
- Channels
- Signal Transduction
- Metabolism
- Drug metabolism
- Cancer
- Drug resistance
- MRP-related proteins
- Cardiovascular
- Atherosclerosis
- Hypertension
- Sodium/ion transport
- Cardiovascular
- Blood
- Blood Pressure regulation
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Hormone biosynthesis
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Drug metabolism
- Metabolism
- Pathways and Processes
- Endocrine metabolism
- Hormone biosynthesis
- Metabolism
- Types of disease
- Heart disease
Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.Tissue specificity
Lung, testis and peripheral blood mononuclear cells.Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.Contains 2 ABC transmembrane type-1 domains.Contains 2 ABC transporter domains.Cellular localization
Cell membrane.- Information by UniProt
Alternative names
- ABC 29
- ABC29
- ABCC
see allDatabase links
- Entrez Gene:4363 Human
- Omim:158343 Human
- SwissProt:P33527 Human
- Unigene:391464 Human
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