TGF Beta FirePlex®-HT Panel
TGF Beta FirePlex®-HT Panel (ab270739) uses FirePlex® particle technology to detect TGF-B1, TGF-B2, and TGF-B3 from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product"s certificate of analysis, which can be foundhere.
FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.
This product is currently only supported for customers in North America and Europe, if you are outside these areas pleasecontact us.
The followingtable provides the sensitivity, dynamic range, and CVs for TGF Beta FirePlex®-HT Panel 1*.
Target | Sensitivity/LOD (pg/ml) | Dynamic Range (pg/ml) | Inter-well CV (Std curve) |
TGF-B1 | 6.39 | 13.72- 3,333 | 6.1% |
TGF-B2 | 48.63 | 123.46- 30,000 | 5.0% |
TGF-B3 | 27.57 | 41.15- 10,000 | 5.7% |
Components | 1 x 384 tests |
---|---|
384 Well Imaging Plate | 1 unit |
384 Well Imaging Plate Lid | 1 unit |
384 Well Imaging Plate Seal | 2 units |
FirePlex-HT 1X PBS | 1 x 8ml |
FirePlex-HT 5X Capture Particle Mix | 1 x 700µl |
FirePlex-HT Protein Standard Mix HTTG | 1 x 7 tests |
FirePlex-HT Reagent Diluent R2 | 1 x 8ml |
FirePlex-HT Sample Diluent S1 | 1 x 25ml |
FirePlex-HT- Imaging Dye Concentrate | 1 x 875µl |
FirePlex-HT35X Detector Antibody Mix | 1 x 110µl |
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