ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.
Components | 1 kit |
---|---|
4X Trypsin Enzyme Concentrate | 1 x 3ml |
Blocking Buffer | 1 x 6ml |
Control Slides (stained) | 1 slide |
Control Slides (unstained) | 4 slides |
DAB Solution 1(DAB Concentrate) | 1 x 0.3ml |
DAB Solution 2(Substrate Reaction Buffer) | 1 x 6ml |
Denaturing Solution | 1 x 6ml |
Detector Antibody (Biotinylated and pre-diluted) | 1 x 6ml |
Hematoxylin Counterstain | 1 x 6ml |
Mounting Media | 1 x 6ml |
Streptavidin-HRP Conjugate (pre-diluted) | 1 x 6ml |
Trypsin Dilution Buffer | 1 x 12ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
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Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。