ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.
Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair ofspots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
A table listing all of ourhuman membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Components | 1 x 4 Membranes | 1 x 8 Membranes |
---|---|---|
1,000X HRP-Conjugated Streptavidin | 1 x 50µl | 1 x 50µl |
1X Blocking Buffer | 1 x 25ml | 2 x 25ml |
2000X Biotin-Conjugated Anti-Cytokines | 1 x 2 vials | 1 x 4 vials |
20X Wash Buffer I | 1 x 10ml | 1 x 20ml |
20X Wash Buffer II | 1 x 10ml | 1 x 20ml |
2X Cell Lysis Buffer | 1 x 10ml | 1 x 16ml |
8-Well Incubation Tray (with Lid) | 1 unit | 1 unit |
Angiogenesis Antibody Array Membranes | 4 units | 8 units |
Detection Buffer C | 1 x 1.5ml | 1 x 2.5ml |
Detection Buffer D | 1 x 1.5ml | 1 x 2.5ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。