Recommended control: Human Wild Type A549 cell line (ab255450)
Please note a wild type cell line is not automatically included with a KO cell line order, if required please add recommended wild type cell line at no additional cost using the code WILDTYPE-TMTK1
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, supplemented with 10% (v/v) DMSO
Handling procedure: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C will result in loss of viability.1. Thaw the vial in 37°C water bath approximately 1-2 minutes.2. Transfer the cell suspension to a 15 mL conical tube with pre-warmed 5 mL complete mediumDMEM+10% FBS, spin 125×g for approximately 5 minutes at room temperature.3. Resuspend the cell pellet with 1 mL pre-warmed complete mediumDMEM+10% FBS and dispense into a 25cm2 culture flask containing 10 mL pre-warmed complete complete mediumDMEM+10% FBS.4. Incubate the culture at 37°C incubator with 5% CO2.5. A subcultivation ratio of 1:4-1:6 is recommended. Cells should be passaged when cells grow splitting at 80-90% confluence.
Click here to view the Mammalian cell tissue culture protocol
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
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Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
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