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产品名称：Abcam//ab7902

产品编号：ab7902

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>聚合物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Abcam

公司：Abcam

公司分类：

商品介绍

Product name
HEK293 whole cell lysateSee all HEK293 lysates
General notes
Cell line: HEK293 (Human embryonic kidney cell line).Growth media: RPMI and 10% FBS (Fetal bovine serum).
293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Tested applications
Suitable for:WBmore details

Mycoplasma free
Yes
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at-80°C. Avoid freeze / thaw cycle.
Storage buffer
Constituent: 5% Beta mercaptoethanol
Concentration information loading...
Lysate notes
293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
- Kits/ Lysates/ Other
- Lysates
- Whole Cell Lysates
- Human
- Other
Background
Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields.

品牌介绍

Abcam 位于英国的剑桥科学园，成立于1998年，专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂，并不断添加，供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品，在2011并购了美国的 MitoSciences 公司，加强了免疫分析方面的产品供应；同年也并购了英国的 Ascent Scientific 公司，开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司，成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录，为各地科学家提供尖端产品，成为各国科学界的重要伙伴，为所有产品提供技术支持来使客户获得预期的结果，为提供高质量的抗体来指向尽可能多的靶蛋白，尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域，包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。

公司介绍

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