Cocktail of primary antibodies to detect apoptosis biomarkers caspase 3 and PARP, along with loading control muscle actin (42 kDa). The caspase 3 antibody (rabbit monoclonal) detects both the 32 kDa pro-caspase 3 as well as the p17 subunit of active caspase 3 generated by cleavage of the pro-caspase 3 at Asp175. The PARP antibody (mouse monoclonal) detects only the apoptosis-specific 89 kDa PARP fragment (cleaved-PARP) generated from the full length PARP by active caspases. Since the primary antibodies used are both mouse and rabbit, a secondary antibodies cocktail of GAM-HRP and GAR-HRP is provided.
The Apoptosis western blot cocktail (ab136812) is designed to study the induction of apoptosis in response to various stimuli. The two main components of this cocktail are monoclonal antibodies specific to caspase 3 and PARP. Caspase 3 is one of the executioner caspases activated by proteolytic cleavage during apoptosis. The rabbit caspase 3 antibody of this cocktail detects both the 32 kDa pro-caspase 3 as well as the p17 subunit of the active caspase 3 generated by cleavage of the pro-caspase 3 at Asp175. Thus the induction of apoptosis can be followed by a decrease of the pro-caspase 3 or by an increase of the p17 caspase 3. Monitoring the changes in the pro-caspase 3 is particularly advantageous, since the proportion of caspase activation can be determined from the reduction of the pro-form from analysis of control and stimulated samples. Poly [ADP-ribose] polymerase 1 (PARP) is a DNA repair enzyme that is cleaved during apoptosis by activated caspases. The mouse PARP antibody of this cocktail detects only the apoptosis-specific 89 kDa PARP fragment (cleaved-PARP). This antibody does not react with the full-length PARP. Combined, these two antibodies provide biomarkers of apoptosis. The rabbit muscle actin antibody is provided as a loading control for sample to sample normalization. Since the primary antibodies are both mouse and rabbit, the cocktail of HRP-conjugated goat anti-rabbit and anti-mouse secondary antibodies is provided for convenience. The targets are easily resolved by Western blot given their different molecular weights.
Components | 200 µl |
---|---|
100X HRP Conjugated Secondary Antibody Cocktail | 1 x 500µl |
250X Primary Antibody Cocktail | 1 x 200µl |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。