ab110411 contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242), Complex II subunit 30kDa (ab14714), Complex III subunit Core 2 (ab14745), Complex IV subunit II (ab110258), and ATP synthase subunit alpha (ab14748) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (Please refer to application notes).

The Western blot cocktail is supplied at a concentration of 1.5 mg/mL.

The five antibodies provided in the cocktail are all mouse monoclonal antibodies.

We recommend using a high pH CAPS buffer / PVDF transfer protocol when using this antibody for Western blot. Please contact us for more details.

Due to a policy change, the previously provided control for this kit has been removed.

• Signal Transduction
• Metabolism
• Mitochondrial

• Kits/ Lysates/ Other
• Kits
• Cell Metabolism Kits
• Other Metabolism Assay

• Metabolism
• Pathways and Processes
• Mitochondrial Metabolism
• Mitochondrial markers

• Kits/ Lysates/ Other
• Kits
• Antibody Cocktails
• OXPHOS