This IHC kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit ab80437, except that it uses the HRP micro-polymer antibody used during the validation of Abcam"s antibodies inIHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate.
The previous version of this kit, the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit, wasused by researchers to stain over 75,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Mouse / anti-Rabbit HRP-DABMicro-polymer IHC Kit ab236466, andHRP-AEC micro-polymer IHC kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in theIHC kits and reagents guide.
Components | 15 ml | 60 ml |
---|---|---|
50x DAB Chromogen | 1 x 0.5ml | 1 x 2ml |
DAB substrate | 1 x 15ml | 1 x 60ml |
Goat anti-rabbit HRP Conjugate | 1 x 15ml | 1 x 60ml |
Hydrogen Peroxide Block | 1 x 15ml | 1 x 60ml |
Protein block | 1 x 15ml | 1 x 60ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
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Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。