Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.
The mouse on mouse kit includes an Fab"anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab"anti-mouse IgG(H+L) detection reagent.
This kit replaces mouse on mouse kit ab127055. The reagents used in this kit have been carefully optimized to match the performance of ab127055 (see validation images below). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent ab64218.
In the mouse on mouse IHC protocol:- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.- Stain with a chromogen (usually DAB, eg. ab64238) for detection.
Otherwise, follow a conventional IHC protocol for DAB staining.
The detector and blocker have <1% reactivity="" to="" human="" and="" rabbit="">1%>
Other products for IHC
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Components | 15 ml | 60 ml |
---|---|---|
HRP polymer detector (ready to use) | 1 x 15ml | 1 x 60ml |
Mouse on mouse blocking reagent (ready to use) | 1 x 15ml | 1 x 60ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。