Rabbit specific HRP/DAB (ABC) IHC Detection Kit ab64261 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:- a sample is incubated with an unconjugated primary antibody specific to the target antigen- a biotinylated secondary antibody then binds to the primary antibody- HRP-labeled streptavidin then binds to the secondary antibody- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.
For alternative streptavidin-biotin IHC detection kits see:- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264 - Anti-Mouse HRP-DAB IHC Kit ab64259
For high sensitivity HRP micro-polymer IHC detection kits see:- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466- Anti-Rabbit HRP-DABMicro-polymer IHC Kit ab236469Alternatively, see HRP-AEC IHC detection kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Components | 15 ml |
---|---|
50x DAB Chromogen | 1 x 0.5ml |
Biotinylated goat anti-rabbit IgG(H+L) | 1 x 15ml |
DAB substrate | 1 x 15ml |
Hydrogen Peroxide Block | 1 x 15ml |
Protein block | 1 x 15ml |
Streptavidin Peroxidase | 1 x 15ml |
Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。在线目录 (www.abcam.cn) 已有超过 120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。
为使研究员更容易找到蛋白质研究试剂产品,在2011并购了美国的 MitoSciences 公司,加强了免疫分析方面的产品供应;同年也并购了英国的 Ascent Scientific 公司,开展了生化试剂的供应。在2012年并购了美国的 Epitomics 公司,成为一家有领导地位的 RabMAbs® 供应商。
Abcam 的目标是给世界上最好的抗体建立最大的在线目录,为各地科学家提供尖端产品,成为各国科学界的重要伙伴,为所有产品提供技术支持来使客户获得预期的结果,为提供高质量的抗体来指向尽可能多的靶蛋白,尽所能在尽可能多的应用和物种中检测每种抗体。
Abcam 专注于特定的研究领域,包括肿瘤研究、心血管研究、染色质和基因调控研究、免疫学、内参对照、微生物学、神经科学、细胞核信号转导研究、RNAi 研究、干细胞研究、亚细胞标记物等。