DRAQ5™ is a cell permeable far-red fluorescent DNA dye that can be used in fixed or non-fixed/ live cells in combination with common labels such as GFP or FITC.
As with any cell-permeant DNA intercalating probe, DRAQ5 may inhibit cell division in long-term assays and should be tested for any effect.
DRAQ5 staining can be used in flow cytometry, live cell imaging and cell-based assays and the dye is highly compatible with standard protocols across many instrumentation platforms.
The chemical name of DRAQ5 is 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione.
The advantages of DRAQ5 staining include- convenient ready-to-use aqueous solution- rapid uptake into living cells, providing a high level of nuclear discrimination- no photobleaching effect- can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.- no compensation needed with common FITC/GFP + PE combinations in flow cytometry- no RNase treatment required- no fluorescence enhancement upon DNA binding- compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes
SPECTRAL PROPERTIES:
Excitation
Emission (instrument dependent):- 665 nm to infra-red max 681 nm / 697 nm intercalated with dsDNA)- minimal overlap with vis range e.g. GFP and FITC- Em. filters may include 695L, 715LP or 780 LP
Concentration: 5 mM
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